A Simple Silver-StainingTechniquefor DetectingBence Jones Proteinsin Unconcentrated Unne

sion to study the time courses of the cytotoxic reaction, some of the cells were stained, but it was difficult to establish target cells (injured cells) from other cells until about 30-60 mm after starting the reaction. Study of L-iO cell lysis with the imaging sensor system showed a slower lysis for GRSL cells: approximately 20 mm until the cell image area reached the minimum. Perhaps there are fewer antigen-antibody binding sites on the L-i0 cells than on GRSL cells, or perhaps the L-iO cell membrane is stronger than that of GRSL. Because the membrane composition of hepatoma cells is similar to that of leukemia cells (10), we favor the former explanation. This new method can become widely applicable to all cytotoxic reactions in addition to complement-mediated cytotoxic reactions reported here. We believe the image sensor system may provide very useful tool for biological research.